据2022报道，近3年全国茶叶抽检报告，农药残留超标占比86.3%，欧盟通报我国出口绿茶农药残留超标，韩国出口红茶被检出农药残留超标，等等农药残留超标困扰事件；农药残留超标问题已成为我国茶叶的一道关卡。
在茶叶农残的检测过程中，其提取物较为复杂，对净化过程要求较高，随着发达国家和我国对茶叶农残限量要求的逐步提高，高效率、低检测限成为茶叶农残检测的趋势。
本文介绍茶叶基质中常见农残测定的高效前处理解决方案，可采用Leowlab Purifier A48正压固相萃取仪实现茶叶复杂基质高效萃取，搭配Leowlab SmartVap AE全自动氮吹浓缩仪使用，无需样品转移，减少样品的损失。
[/fusion_text][fusion_builder_row_inner][fusion_builder_column_inner type=”1_3″ type=”1_3″ align_self=”auto” content_layout=”column” align_content=”flex-start” valign_content=”flex-start” content_wrap=”wrap” center_content=”no” column_tag=”div” target=”_self” hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” order_medium=”0″ order_small=”0″ hover_type=”none” border_style=”solid” box_shadow=”no” box_shadow_blur=”0″ box_shadow_spread=”0″ background_type=”single” gradient_start_position=”0″ gradient_end_position=”100″ gradient_type=”linear” radial_direction=”center center” linear_angle=”180″ lazy_load=”avada” background_position=”left top” background_repeat=”no-repeat” background_blend_mode=”none” background_slider_skip_lazy_loading=”no” background_slider_loop=”yes” background_slider_pause_on_hover=”no” background_slider_slideshow_speed=”5000″ background_slider_animation=”fade” background_slider_direction=”up” 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filter_sepia_hover=”0″ filter_opacity_hover=”100″ filter_blur_hover=”0″ image_id=”6065|fusion-400″ link=”https://leowlab.com/positive-pressure-solid-phase-extractor/purifier-a48/” caption_title=”Leowlab Purifier A48″ caption_text=”48位正压固相萃取仪” caption_title_transform=”capitalize” caption_text_transform=”capitalize” caption_overlay_color=”hsla(var(–awb-color7-h),var(–awb-color7-s),calc(var(–awb-color7-l) + 40%),var(–awb-color7-a))” fusion_font_variant_caption_title_font=”600″ fusion_font_family_caption_title_font=”Montserrat” caption_title_size=”16″ caption_title_color=”var(–awb-color1)” fusion_font_variant_caption_text_font=”400″ fusion_font_family_caption_text_font=”Montserrat” caption_text_size=”16″ caption_border_color=”var(–awb-color1)” caption_text_color=”var(–awb-color1)”]https://leowlab.com/wp-content/uploads/2025/10/A48-400×400.jpg[/fusion_imageframe][/fusion_builder_column_inner][fusion_builder_column_inner type=”1_3″ type=”1_3″ align_self=”auto” content_layout=”column” 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filter_hover_element=”self” filter_hue=”0″ filter_saturation=”100″ filter_brightness=”100″ filter_contrast=”100″ filter_invert=”0″ filter_sepia=”0″ filter_opacity=”100″ filter_blur=”0″ filter_hue_hover=”0″ filter_saturation_hover=”100″ filter_brightness_hover=”100″ filter_contrast_hover=”100″ filter_invert_hover=”0″ filter_sepia_hover=”0″ filter_opacity_hover=”100″ filter_blur_hover=”0″ transform_type=”regular” transform_hover_element=”self” transform_scale_x=”1″ transform_scale_y=”1″ transform_translate_x=”0″ transform_translate_y=”0″ transform_rotate=”0″ transform_skew_x=”0″ transform_skew_y=”0″ transform_scale_x_hover=”1″ transform_scale_y_hover=”1″ transform_translate_x_hover=”0″ transform_translate_y_hover=”0″ transform_rotate_hover=”0″ transform_skew_x_hover=”0″ transform_skew_y_hover=”0″ transition_duration=”300″ transition_easing=”ease” scroll_motion_devices=”small-visibility,medium-visibility,large-visibility” animation_direction=”left” animation_speed=”0.3″ animation_delay=”0″ last=”no” border_position=”all”][fusion_imageframe custom_aspect_ratio=”100″ lightbox=”no” linktarget=”_blank” align_medium=”none” align_small=”none” align=”center” hover_type=”none” magnify_duration=”120″ scroll_height=”100″ scroll_speed=”1″ caption_style=”resa” caption_align_medium=”none” caption_align_small=”none” caption_align=”center” caption_title_tag=”div” animation_direction=”left” animation_speed=”0.3″ animation_delay=”0″ hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” filter_hue=”0″ filter_saturation=”100″ filter_brightness=”100″ filter_contrast=”100″ filter_invert=”0″ filter_sepia=”0″ filter_opacity=”100″ filter_blur=”0″ filter_hue_hover=”0″ filter_saturation_hover=”100″ filter_brightness_hover=”100″ filter_contrast_hover=”100″ filter_invert_hover=”0″ filter_sepia_hover=”0″ filter_opacity_hover=”100″ filter_blur_hover=”0″ image_id=”6069|fusion-400″ link=”https://leowlab.com/nitrogen-evaporator/smartvap-ae/” caption_title=”Leowlab SmartVap AE” caption_title_transform=”capitalize” caption_text_transform=”capitalize” caption_overlay_color=”hsla(var(–awb-color7-h),var(–awb-color7-s),calc(var(–awb-color7-l) + 40%),var(–awb-color7-a))” fusion_font_variant_caption_title_font=”600″ fusion_font_family_caption_title_font=”Montserrat” caption_title_size=”16″ caption_title_color=”var(–awb-color1)” fusion_font_variant_caption_text_font=”400″ fusion_font_family_caption_text_font=”Montserrat” caption_text_size=”16″ caption_border_color=”var(–awb-color1)” caption_text=”全自动氮吹浓缩仪”]https://leowlab.com/wp-content/uploads/2025/10/QE-AE-400×400.jpg[/fusion_imageframe][/fusion_builder_column_inner][fusion_builder_column_inner type=”1_3″ type=”1_3″ align_self=”auto” content_layout=”column” align_content=”flex-start” valign_content=”flex-start” content_wrap=”wrap” center_content=”no” column_tag=”div” target=”_self” 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filter_sepia=”0″ filter_opacity=”100″ filter_blur=”0″ filter_hue_hover=”0″ filter_saturation_hover=”100″ filter_brightness_hover=”100″ filter_contrast_hover=”100″ filter_invert_hover=”0″ filter_sepia_hover=”0″ filter_opacity_hover=”100″ filter_blur_hover=”0″ transform_type=”regular” transform_hover_element=”self” transform_scale_x=”1″ transform_scale_y=”1″ transform_translate_x=”0″ transform_translate_y=”0″ transform_rotate=”0″ transform_skew_x=”0″ transform_skew_y=”0″ transform_scale_x_hover=”1″ transform_scale_y_hover=”1″ transform_translate_x_hover=”0″ transform_translate_y_hover=”0″ transform_rotate_hover=”0″ transform_skew_x_hover=”0″ transform_skew_y_hover=”0″ transition_duration=”300″ transition_easing=”ease” scroll_motion_devices=”small-visibility,medium-visibility,large-visibility” animation_direction=”left” animation_speed=”0.3″ animation_delay=”0″ last=”no” border_position=”all”][fusion_imageframe custom_aspect_ratio=”100″ lightbox=”no” linktarget=”_blank” align_medium=”none” align_small=”none” align=”center” hover_type=”none” magnify_duration=”120″ scroll_height=”100″ scroll_speed=”1″ caption_style=”resa” caption_align_medium=”none” caption_align_small=”none” caption_align=”center” caption_title_tag=”div” animation_direction=”left” animation_speed=”0.3″ animation_delay=”0″ hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” filter_hue=”0″ filter_saturation=”100″ filter_brightness=”100″ filter_contrast=”100″ filter_invert=”0″ filter_sepia=”0″ filter_opacity=”100″ filter_blur=”0″ filter_hue_hover=”0″ filter_saturation_hover=”100″ filter_brightness_hover=”100″ filter_contrast_hover=”100″ filter_invert_hover=”0″ filter_sepia_hover=”0″ filter_opacity_hover=”100″ filter_blur_hover=”0″ image_id=”6071|fusion-400″ link=”https://leowlab.com/nitrogen-generator/nitrogen-evaporator-1/clart-mp-series/” caption_title=”Leowlab NG-MP” caption_text=”氮吹用氮气发生器” caption_title_transform=”capitalize” caption_text_transform=”capitalize” caption_overlay_color=”hsla(var(–awb-color7-h),var(–awb-color7-s),calc(var(–awb-color7-l) + 40%),var(–awb-color7-a))” fusion_font_variant_caption_title_font=”600″ fusion_font_family_caption_title_font=”Montserrat” caption_title_size=”16″ caption_title_color=”var(–awb-color1)” fusion_font_variant_caption_text_font=”400″ fusion_font_family_caption_text_font=”Montserrat” caption_text_size=”16″ caption_border_color=”var(–awb-color1)”]https://leowlab.com/wp-content/uploads/2025/10/氮发-中-400×400.jpg[/fusion_imageframe][/fusion_builder_column_inner][/fusion_builder_row_inner][fusion_title title_type=”text” scroll_reveal_effect=”color_change” scroll_reveal_basis=”chars” scroll_reveal_behavior=”always” scroll_reveal_duration=”500″ scroll_reveal_stagger=”200″ scroll_reveal_delay=”0″ scroll_reveal_above_fold=”yes” marquee_direction=”left” marquee_mask_edges=”no” marquee_speed=”15000″ rotation_effect=”bounceIn” display_time=”1200″ highlight_effect=”circle” loop_animation=”once” highlight_animation_duration=”1500″ highlight_width=”9″ highlight_smudge_effect=”no” highlight_top_margin=”0″ title_link=”off” link_target=”_self” content_align=”left” size=”div” text_shadow=”no” text_shadow_blur=”0″ text_stroke=”no” text_stroke_size=”1″ text_overflow=”none” gradient_font=”no” gradient_start_position=”0″ gradient_end_position=”100″ gradient_type=”linear” radial_direction=”center center” linear_angle=”180″ style_type=”default” animation_direction=”left” animation_speed=”0.3″ animation_delay=”0″ hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” font_size=”30″ fusion_font_variant_title_font=”600″ fusion_font_family_title_font=”Montserrat” margin_bottom=”30px” margin_top=”30px”]仪器设备
[/fusion_title][fusion_table fusion_table_type=”2″ fusion_table_rows=”4″ fusion_table_columns=”2″ animation_direction=”left” animation_speed=”0.3″ animation_delay=”0″ hide_on_mobile=”small-visibility,medium-visibility,large-visibility”]| 前处理仪器设备 | |
|---|---|
| 萃取仪 | Leowlab Purifier A48正压固相萃取仪 |
| 浓缩系统 | Leowlab SmartVap AE全自动氮吹浓缩仪 |
| 搭配浓缩系统仪器 | Leowlab NG-MP系列氮吹用氮气发生器 |
| 分析仪器 | |
|---|---|
| 气相色谱仪 | 配有双火焰光度检测器(FPD磷滤光片) |
前处理流程图
[/fusion_title][fusion_imageframe custom_aspect_ratio=”100″ lightbox=”no” linktarget=”_self” align_medium=”none” align_small=”none” align=”none” hover_type=”none” magnify_duration=”120″ scroll_height=”100″ scroll_speed=”1″ caption_style=”off” caption_align_medium=”none” caption_align_small=”none” caption_align=”none” caption_title_tag=”2″ animation_direction=”left” animation_speed=”0.3″ animation_delay=”0″ hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” filter_hue=”0″ filter_saturation=”100″ filter_brightness=”100″ filter_contrast=”100″ filter_invert=”0″ filter_sepia=”0″ filter_opacity=”100″ filter_blur=”0″ filter_hue_hover=”0″ filter_saturation_hover=”100″ filter_brightness_hover=”100″ filter_contrast_hover=”100″ filter_invert_hover=”0″ filter_sepia_hover=”0″ filter_opacity_hover=”100″ filter_blur_hover=”0″ image_id=”7427|full”]https://leowlab.com/wp-content/uploads/2026/01/茶叶.png[/fusion_imageframe][fusion_title title_type=”text” scroll_reveal_effect=”color_change” scroll_reveal_basis=”chars” scroll_reveal_behavior=”always” scroll_reveal_duration=”500″ scroll_reveal_stagger=”200″ scroll_reveal_delay=”0″ scroll_reveal_above_fold=”yes” marquee_direction=”left” marquee_mask_edges=”no” marquee_speed=”15000″ rotation_effect=”bounceIn” display_time=”1200″ highlight_effect=”circle” loop_animation=”once” highlight_animation_duration=”1500″ highlight_width=”9″ highlight_smudge_effect=”no” highlight_top_margin=”0″ title_link=”off” link_target=”_self” content_align=”left” size=”div” text_shadow=”no” text_shadow_blur=”0″ text_stroke=”no” text_stroke_size=”1″ text_overflow=”none” gradient_font=”no” gradient_start_position=”0″ gradient_end_position=”100″ gradient_type=”linear” radial_direction=”center center” linear_angle=”180″ style_type=”default” animation_direction=”left” animation_speed=”0.3″ animation_delay=”0″ hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” font_size=”26″ fusion_font_variant_title_font=”600″ fusion_font_family_title_font=”Montserrat” margin_bottom=”30px” margin_top=”30px”]实验方法概要：
[/fusion_title][fusion_text animation_direction=”left” animation_speed=”0.3″ animation_delay=”0″ disable_idd=”no” hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” fusion_font_variant_text_font=”400″ fusion_font_family_text_font=”Montserrat” columns=”1″]1. 称取5g茶叶，加入乙腈提取，加NaCl除水；
2. 采用Leowlab SmartVap AE全自动氮吹浓缩仪在80℃下，按程序自动将样品氮吹近干；
3. 加乙腈-甲苯溶解残余物，采用Leowlab Purifier A48正压固相萃取仪进行净化处理；
4. 将（固相萃取仪配套的）过柱后的收集管直接放至Leowlab SmartVap AE全自动氮吹浓缩仪，80℃水浴，设置程序，自动氮吹近干。
5. 用1.00ml丙酮复溶样品，涡旋混合，待上机。
[/fusion_text][fusion_title title_type=”text” scroll_reveal_effect=”color_change” scroll_reveal_basis=”chars” scroll_reveal_behavior=”always” scroll_reveal_duration=”500″ scroll_reveal_stagger=”200″ scroll_reveal_delay=”0″ scroll_reveal_above_fold=”yes” marquee_direction=”left” marquee_mask_edges=”no” marquee_speed=”15000″ rotation_effect=”bounceIn” display_time=”1200″ highlight_effect=”circle” loop_animation=”once” highlight_animation_duration=”1500″ highlight_width=”9″ highlight_smudge_effect=”no” highlight_top_margin=”0″ title_link=”off” link_target=”_self” content_align=”left” size=”div” text_shadow=”no” text_shadow_blur=”0″ text_stroke=”no” text_stroke_size=”1″ text_overflow=”none” gradient_font=”no” gradient_start_position=”0″ gradient_end_position=”100″ gradient_type=”linear” radial_direction=”center center” linear_angle=”180″ style_type=”default” animation_direction=”left” animation_speed=”0.3″ animation_delay=”0″ hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” font_size=”30″ fusion_font_variant_title_font=”600″ fusion_font_family_title_font=”Montserrat” margin_bottom=”30px” margin_top=”30px”]结论[/fusion_title][fusion_text animation_direction=”left” animation_speed=”0.3″ animation_delay=”0″ disable_idd=”no” hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” fusion_font_variant_text_font=”400″ fusion_font_family_text_font=”Montserrat” columns=”1″]茶叶是比较复杂的基质，在前处理过程，利用半自动正压固相萃取仪代替负压萃取装置，大大减少前处理时间，样品均匀过柱，获得极佳的重复性和回收率。
[/fusion_text][fusion_title title_type=”text” scroll_reveal_effect=”color_change” scroll_reveal_basis=”chars” scroll_reveal_behavior=”always” scroll_reveal_duration=”500″ scroll_reveal_stagger=”200″ scroll_reveal_delay=”0″ scroll_reveal_above_fold=”yes” marquee_direction=”left” marquee_mask_edges=”no” marquee_speed=”15000″ rotation_effect=”bounceIn” display_time=”1200″ highlight_effect=”circle” loop_animation=”once” highlight_animation_duration=”1500″ highlight_width=”9″ highlight_smudge_effect=”no” highlight_top_margin=”0″ title_link=”off” link_target=”_self” content_align=”left” size=”div” text_shadow=”no” text_shadow_blur=”0″ text_stroke=”no” text_stroke_size=”1″ text_overflow=”none” gradient_font=”no” gradient_start_position=”0″ gradient_end_position=”100″ gradient_type=”linear” radial_direction=”center center” linear_angle=”180″ style_type=”default” animation_direction=”left” animation_speed=”0.3″ animation_delay=”0″ hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” font_size=”30″ fusion_font_variant_title_font=”600″ fusion_font_family_title_font=”Montserrat” margin_bottom=”30px” margin_top=”30px”]方案特点
[/fusion_title][fusion_text animation_direction=”left” animation_speed=”0.3″ animation_delay=”0″ disable_idd=”no” hide_on_mobile=”small-visibility,medium-visibility,large-visibility” sticky_display=”normal,sticky” fusion_font_variant_text_font=”400″ fusion_font_family_text_font=”Montserrat” columns=”1″]1. 48个样同时过柱：Leowlab Purifier A48正压固相萃取仪可一次性同时处理48个样品，大大减少实验时间；
2. 复杂基质，轻松过柱：针对复杂的基质茶叶，正压最大承受压力100psi,远超过负压，轻松更快过柱；
3. 很好的重复性和回收率：氮气作为气源，正压过柱，保证每个柱子压力均匀性，保证极佳的回收率；
4. 净化到浓缩收集管直接转移：全自动氮吹浓缩仪，接收管体积0-40mL，净化完的接收管可直接转移至Leowlab SmartVap AE全自动氮吹浓缩仪氮吹，减少样品在转移过程中的损失。
5. 涡旋斜吹技术：Leowlab SmartVap AE采用涡旋剪切气流技术，样品浓缩快速、平和；
6. 智能化浓缩：Leowlab SmartVap AE气流可自动控制，支持梯度自动控制，优化浓缩进程；
7. 多种支架可供选择：Leowlab SmartVap AE适应不同直径不同高度的试管、离心管。
参考文献
[1] 李军明,钟读波,王亚琴等.茶叶中农药残留限量及检测方法研究进展[J] 云南大学学报 (自然科学版)，2010，32 (S1): 299～304 [/fusion_text][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]